Applications

Physiology / Whole organs

Whole Mouse Brain

PEGASOS cleared whole mouse brain, Life-act EGFP transgenic actin filaments staining, tdTomato transgenic MARCKS plasma membrane staining. Objective: 4X/0.28NA/30WD.

Courtesy of Pr. Edna Hardeman, Bio-Imaging Facility (BMIF) of UNSW Sydney.

Human Gastric Organoids

Human gastric organoids in PBS1X, with nuclear DAPI staining and membrane CDH1/Alexa546 Staining. Objective: 40X/0.80NA/3.3WD.

Courtesy of Elena Amendola, Monte S. Angelo University Federico II.

Zebrafish Larva, Multi-Scale Imaging

CUBIC R+ (RI 1.52) cleared whole zebrafish larva (10.5 x 3 x 2.2 mm), YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Large Scale Imaging, illumination objective: 4X (UPLFLN4X), detection objective: 4X (XLFLUOR4X/340). Small-Scale Imaging, illumination objective: 10X, detection objective: 10X.

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Pig Brain, Multi-Scale Imaging

iDisco cleared 2×2 stitched pig brain imaging at 638nm illumination, emission filter at 690/50 nm. Large Scale Imaging, illumination objective: 4X (UPLFLN4X), detection objective: 4X (XLFLUOR4X/340). Small-Scale Imaging, illumination objective: 10X (LMPLFLN10X), detection objective: 10X (XLPLN10XSVMP-2).

Courtesy of Dr. Alexander Rapp, TU Darmstadt, Germany.

Cleared Brain Tissue

CCK+interneurons in the entorhino-hippocampal formation in the mouse brain (GFP) and Wfs1-marked pyramidal. Objective: Olympus XLFLUOR4X/340 (4X/0.28NA/30WD).

Courtesy of Nóra Henn-Mike, Institute of Physiology, Medical School, University of Pécs.

Zebrafish Brain

Cleared whole zebrafish brain, DAPI staining, Alexa 488 neuronal staining, DiD neuronal staining. Objective: Olympus XLPLN10XSVMP-2 MPE (10X/0.60NA/8WD).

Courtesy of Isabelle Robineau & Matthieu Simion, TEFOR Paris-Saclay.

CUBIC R+ cleared whole Zebrafish Larva

CUBIC R+ cleared whole Zebrafish Larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective, UPLFLN4X (4X/0.28NA/30WD).

Courtesy of Dr. Matthieu Simion (TEFOR Paris-Saclay).

CUBIC R+ cleared whole Zebrafish Larva by PhaseView Alpha3 Light Sheet Microscope

Whole mouse brain

PEGASOS cleared whole mouse brain, Life-act EGFP transgenic actin filaments staining, tdTomato transgenic MARCKS, plasma membrane staining. Objective, UPLFLN4X (4X/0.28NA/30WD).

Courtesy of Pr. Edna Hardeman and Bio-Imaging Facility (BMIF) of UNSW Sydney.

Whole mouse brain

PEGASOS cleared whole mouse brain, Life-act EGFP transgenic actin filaments staining, tdTomato transgenic MARCKS, plasma membrane staining. Objective, UPLFLN4X (4X/0.28NA/30WD).

Courtesy of Dr. Genaro Hernandez, University of Texas Southwestern, Dallas, USA.

Whole mouse brain by PhaseView Alpha3 Light Sheet Microscope

Retina / Morphological analysis

Mouse Retina

Retinal whole mount from mouse with Thy1-GCAMP3. Objective: Olympus XLFLUOR4X/340 (4X/0.28NA/30WD).

Courtesy of Tamas Kovács-Öller, Szentágothai Research Centre (SzKK).

Physiology / Morphological analysis

Zebrafish Larva

CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLSLPN25XGMP (25X/1.00NA/8WD).

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Zebrafish Larva

Cleared whole zebrafish larva, Alexa 488 post-synaptic staining, Alexa 555 glial cells staining. Objective: Olympus XLPLN10XSVMP-2 MPE (10X/0.60NA/8WD).

Courtesy of Elodie Machado & Matthieu Simion / TEFOR Paris-Saclay.

Zebrafish Larva

CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLFLUOR4X/340 (4X/0.28NA/30WD).

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Zebrafish Larva

CUBIC R+ cleared whole zebrafish larva, YOYO-1 nuclear staining, DiD lipophilic staining, Alexa 647 anti-TH dopaminergic neurons staining. Objective: Olympus XLPLN10XSVMP-2 MPE (10X/0.6NA/8WD).

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Multiscale Imaging of Adult Zebrafish

Multiscale Imaging of Adult Zebrafish. Objectives: 4X 0.28NA, 10X 0.6NA.

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Axolotl

ECI-transparized Axolotl Imaging in two channels (RFP, far red) with 10X NA 0.6 objective.

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

Brain / Neuronal activation mapping

Cleared Mouse Brain

iDisco cleared brain with CFOS marking in green channel. Objective: Olympus UPLFLN4X (4X/0.28NA/30WD).

Courtesy of Dr. Genaro Hernandez, University of Texas Southwestern, Dallas, USA.

Zebrafish Brain Imaging

Adult Zebrafish Brain imaged by PhaseView Alpha3 Light Sheet Microscope, at 10X/NA0.6.

Courtesy of Matthieu Simion, TEFOR Paris-Saclay.

© 2021 PhaseView

Developmental Biology

Ganglion Imaging

Ganglion Imaging by PhaseView Alpha3 Light Sheet Microscope.

© 2016 PhaseView

Mouse Embryo Imaging

Mouse embryo in the E11.5 state transparized with CUBIC-L/R. Muscular system (red), nervous system (white).

Courtesy of Eglantine Heude and Frida Sanchez Garrido, Museum National d’Histoire Naturelle, France.

© 2024 PhaseView

Zebrafish Egg

15 hours-timelapse of a zebrafish egg with endothelial GFP staining and transmited light.

Courtesy of Beatriz Novoa & Antonio Figueras (Inmunology and Genomics Lab, Institute of Marine Research.

Astyanax Morpho Development Imaging

Astyanax Morpho Development, 28 hours time lapse, mCherry nuclei staining, GFP cytoplasmic staining in head.

© 2023 PhaseView

Drosophila Imaging

Drosophila fly egg, RFP nuclei staining, GFP membrane staining. Objective: 60X NA/1.0 water.

© 2023 PhaseView

Zebrafish Embryo Imaging

Zebrafish Embryo 36 hpf, RFP / GFP staining.

© 2023 PhaseView

Cell Biology

tumor spheroids

Imaging of tumor spheroids in far red.

Courtesy of Dr. Simona Mura, Institut Galien Paris-Saclay, France.

© 2023 PhaseView

Zebrafish Cellular Division

Imaging of Zebrafish Cellular Division, Nuclei NLS, H2B mCherry staining.

Courtesy of Nadine Peyrieras, BioEmergences, Gif sur Yvette, France.

© 2023 PhaseView

High Speed Imaging

Blood Cell Flow

Imaging of Blood Cell Flow in Zebrafish.

These rapid circulatory movements in live zebrafish were captured for only a few seconds.

© 2023 PhaseView

Red Core Imaging

This example of high-speed imaging utilizing the Alpha3 Light Sheet Microscope illustrates that camera performance is the sole limiting factor for the 3D acquisition speed of the 3D stack, while the sample remains stationary. The scanning range (100 mm/mag²) is covered in under 10 ms.

© 2023 PhaseView

Real Time Heartbeat Observation

Real Time Heartbeat Observation through oculars of PhaseView Alpha3 Light Sheet Microscope.

Real-time observation of phenomena such as the heartbeat is possible through oculars attached to the Alpha3 Light Sheet Microscope.

© 2018 PhaseView

Time Lapse Imaging

This example of high-speed imaging utilizing the Alpha3 Light Sheet Microscope shows a 52-minute recording time lapse imaging with 60X objective.

© 2016 PhaseView

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